Next-generation Sequencing (NGS) - NovaSeq 6000 Illumina and MiSeq sequencing platforms.
Two primary sequencing platforms are available to meet various needs. The core team performs library preparations for optimal cluster generation and maximal data output across flow cells to cover a wide range of sequencing needs including targeted sequencing, RNA-seq, 16S metagenomics, methylation and whole genome sequencing. Alternatively, researchers can submit user prepared libraries for quality control and sequencing.
|Flow Cell Type||SP||S1||S2||S4|
|Reads||650-800 M||1.3 - 1.6 B||3.3 - 4.1 B||8 - 10 B|
|Cycle Lengths||100, 300, 500||100, 200, 300||100, 200, 300||200, 300|
|Flow Cell Type||Nano||Micro||V2||V3|
|Reads||1 M||4 M||15 M||25 M|
|Cycle Lengths||300, 500||300||36, 50, 300, 500||100, 600|
Methylation microarray services (human samples) - The MethylationEPIC BeadChip is ideal for human epigenome wide association studies (EWAS). Each chip interrogates over 850,000 methylation sites providing comprehensive single-nucleotide resolution of 99% of RefSeq genes, 95% of CpG islands and a high coverage of enhancer regions.
Single cell sequencing - 10x Genomics Chromium System employing Next GEM Reagents and Chips
SNP genotype analysis ""pre-designed and custom TaqManÂ® SNP Genotyping Assays are run on the Quantstudio 12K Flex platform
TaqManÂ® Gene Expression Assays - real-time Polymerase Chain Reaction (RT-PCR) gene expression analysis are run on the Quantstudio 12K Flex platform
Cell line authentication ""Genetic authentication of human cell lines using short tandem repeat (STR) analysis with Promega's GenePrint 10 system on the Applied Biosystems 3730XL DNA Analyzer.